본문
Lee, Seung Rock
| Department | Biochemistry |
|---|---|
| Major Field of Research | Redox regulation of cell signaling, Redox regulation of the tumor suppressor PTEN and PTP, Enzyme activity and function of selenoproteins |
| leesr@jnu.ac.kr | |
| Homepage |
Research interests
Our Lab general interest is to understand the mechanisms by which organisms maintain redox homeostasis. Main research projects are
1. Redox regulation of PTEN and PTPs in cell signaling and diseases.
2.
Enzyme
activity and functions of selenoproteins mTrxR and Sel O.
3. Physiological functions and application of ascorbic acid glycosides.
Publication
- 1. Kim Y-R, Yu S-W, Lee S-R, Hwang Y-L, Kang S-O. A heme-containing ascorbate oxidase from Pleurotus ostreatus. J. Biol. Chem. 1996;271:3105-3111.
- 2. Lee S-R, Kwon K-S, Kim S.-R, Rhee S. G. Reversible inactivation of protein tyrosine phosphatase 1B in A431 cells stimulated with epidermal growth factor. J. Biol. Chem. 1998;273: 15366-15372.
- 3. Lee S-R, Kim J-R, Kwon K-S, Yoon H-W, Levine R. L, Ginsburg A, Rhee S. G. Molecular cloning and characterization of a mitochondrial selenocysteine-containing thioredoxin reductase from rat liver. J. Biol. Chem. 1999;274:4722-4734.
- 4. Lee S-R, Bar-Noy S, Kwon J, Levine R. L, Stadtman T. C, Rhee S. G. Mammalian thioredoxin reductase : Oxidation of the C-terminal cysteine/selenocysteine active site forms a thioselenide, and replacement of selenium with sulfur markedly reduces catalytic activity. Proc. Natl. Acad. Sci. 2000;97: 2521-2526.
- 5. Lee S-R, Yang K-S, Kwon J, Lee C, Jeong W, Rhee S. G. Reversible inactivation of the tumor suppressor PTEN by H2O2. J. Biol. Chem. 2002;277: 20336-20342.
- 6. Cho S-H, Lee C-H, Ahn Y, Kim H, Kim H, Ahn C-Y, Yang K-S, Lee S-R. Redox regulation of PTEN and protein tyrosine phosphatase in H2O2-mediated cell signaling. FEBS Letters 2004;560:7-13.
- 7. Seo J. H, Yeo C. Y, Lee S-R, Ahn Y, Hur K. C. The major target of the endogenously generated reactive oxygen species in response to insulin stimulation is PTEN not PI-3 kinase. Mol. Biol. Cell. 2005;16: 348 – 357.
- 8. Lee S-R, Kwon J, Yang K-S, ahn Y, Kim Y. J, Earl R. S. Rhee S. G. Reversible oxidation and inactivation of the tumor suppressor PTEN in cells stimulated with peptide growth factors. Proc. Natl. Acd. Sci. 2004;101:16419 –16424 (Co-first author)
- 9. Kim J-H, Lee S-R. Evidence for presence of thioredoxin reductase in nucleus of HeLa cell. Kor. J. Gerontol. 2004;14:33-37.
- 10. Ahn Y, Hwang C. Y, Lee S-R, Kwon K-S, Lee C. The tumor suppressor PTEN mediates a negative regulation of the E3 ubiquitin-protein ligase Nedd4. Biochem. J. 2008;412:331-338
- 11. Lee S-R, Joo W-J, Baek Y-U, Lee Y-K, Yu S-W, Kim Y-R, Chay K-O, Cho S-H, Kang S-O. Intracellular substrates of a heme-containing ascorbate oxidase in Pleurotus ostreatus. J. Microbiol. 2009;47:178-186.
- 12. Kim Y, Song Y. B, Kim T-Y, Kim I, Han S-J, Ahn Y, Cho S-H, Choi C. Y, Chay K-O, Yang S. Y, Ahn B. W, Huh W-K, Lee, S-R. Redox regulation of the tumor suppressor PTEN by glutathione. FEBS Letters 2010;584:3550-3556.
- 13. Lee S-R, Joo W-J, Baek Y-U, Kim I, Chay K-O, Cho S-H, Lee S-J, Kang S-O. Effect of light and reductones on differentiation of Pleurotus ostreatus. J. Microbiol. 2011;49:71-77.
- 14. Kim Y, Chay K-O, Kim I, Song Y. B, Kim T-Y, Han S-J, Ahn Y, Cho S-H, Hoe K-L, Ahn B. W, Lee S-R. Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4. Biochem. Biophys. Res. Comm. 2011;407:175-180.
- 15. Kim I, Han S-J, Ahn Y, Chay K-O, Lee S-R. Tyr740 and Tyr751 residues of platelet-derived growth factor beta receptor are responsible for the redox regulation of phosphatase and tensin homolog in the cells stimulated with platelet-derived growth factor. Redox Rep. 2011, 16: 181-186.
- 16. Han S-J, Lee BC, Yim SH, Gladyshev VN, Lee S-R. Characterization of mammalian selenoprotein O: a redox-active mitochondrial protein. PLoS One, 2014; 9(4):e95518.
- 17. Han S-J, Ahn Y, Park I, Zhang Y, Kim I, Kim HW, Ku CS, Chay KO, Yang SY, Ahn BW, Jang DI, Lee S.-R. Assay of the redox state of the tumor suppressor PTEN by mobility shift. Methods, 2015; 77-78:58-62
- 18. Zhang Y, Han SJ, Park I, Kim I, Chay KO, Kim SM, Jang DI, Lee TH, Lee SR. Redox regulation of the tumor suppressor PTEN by hydrogen peroxide and tert-butyl hydroperoxide. (2017, 05) Int. J. Mol. Sci. 10;18(5)
- 19. Han SJ, ZhangY, Kim I, Chay KO, Yoon HJ, Jang DI, Yang SY, Park J, Woo HA, Park I, Lee SR., Redox regulation of the tumor suppressor PTEN by the thioredoxin system and cumene hydroperoxide. (2017, 11) Free Radic. Biol. Med. 112; 277-286.
- 20. Zhang Y, Park J, Han SJ, Lim Y, Park I, Kim JS, Woo HA, Lee SR. Peroxiredoxin III protects tumor suppressor PTEN from oxidation by 15-Hydroperoxy-eicosatetraenoic acid. (2019, 9) Oxidative Medicine and Cellular Longevity. 2019;2828493-2828500.
- 21. Zhang Y, Park J, Han SJ, Yang SY, Yoon HJ, Park I, Woo HA, Lee SR. Redox regulation of tumor suppressor PTEN in cell signaling. (2020, 5) Redox Biology. 34; 101553.
- 22. Zhang Y, Roh YJ, Han SJ, Park I, Lee HM, Ok YS, Lee BC, Lee SR. Role of selenoproteins in redox regulation of signaling and the antioxidant system: A Review. (2020, 5) Antioxidants 9, 383;antiox9050383.
- 23. Zhang Y, Park J, Han SJ, Lim Y, Park I, Thang NH, Kim JS, Woo HA, Lee SR. The critical role of redox regulation of PTEN and peroxiredoxin III in alcoholic fatty liver. (2020, 11) Free Radic. Biol. Med. 162; 141-148.
- 24. Lee HM, Choi DW, Kim S, Lee A, Kim M, Roh YJ, Jo YH, Cho HY, Lee HJ, Lee SR, Tarrago L, Gladyshev VN, Kim JH, Lee BC. Biosensor-linked immunosorbent assay for the quantification of methionine oxidation in target proteins. (2021, 12) ACS Sensors. 1c01819.
- 25. Thang NH, Park J, Zhang Y, Park I, Yoon HJ, Woo HA, Lee SR. Redox regulation of PTEN by peroxiredoxins. (2021, 2) 10, 302;antiox10020302.