본문
| Department | Chemistry |
|---|---|
| Major Field of Research | enzymology, regulation of metabolic pathways |
| jungch@jnu.ac.kr | |
| Homepage |
Research interests
Che-Hun Jung’s lab studies regulation of proteins and metabolic pathways in E. coli and mammals.
1. ppGpp-binding proteins
- ppGpp serves as an alarmone in stringent responses in various microbes. ppGpp regulates replication, transcription, translation and metabolic pathways.
- ppGpp-immobilized columns were constructed and ppGpp-binding proteins were isolated by affinity chromatography. The ppGpp-binding proteins were identified by a mass spectroscopy.
- A method to determine the binding affinity of ppGpp to proteins was developed.
- Studies on the effect of ppGpp binding to various proteins such as cyclic AMP receptor proteins (CRP), histone-like nucleoid structuring protein (H-NS), stringent starvation protein A (SspA), HU alpha (HupA) and proteases are currently undergoing.
2. Proteins regulated by ascorbic acid
- Only a few proteins are reported as regulated by ascorbic acid.
- A group of proteins, potentially regulated by ascorbic acid, have been isolated and identified by proteomics approaches.
- Studies on the effect of ascorbic acid to various enzymes are underway.
3. Glutathione-binding proteins
- Glutathione (GSH) is the most abundant non-protein thiol in cells. The concentration of GSH has been reported as 17 mM in E. coli when it is grown in glucose-rich media.
- For those ligands with high cellular concentrations such as GSH, the dissociation constant of 0.5 mM (about 3 % of the cellular GSH concentration) will be sufficient for occupying all of the binding sites. A method to measure the weak bindings has been developed and the binding affinities of GSH to various proteins have been reported.
- Studies of the affect of GSH binding to proteins are currently performed.
4. DNA-binding proteins
- Many DNA-binding proteins, which regulate gene expression, have been reported.
- A method to quantitate the binding affinity of proteins to DNA has been developed.
- The binding specificity determined by this study may be useful for elucidating the physiological roles of the DNA-binding proteins.
<그림 최대 3개 (각 파일 사이즈 1M 이상)>
Binding of ppGpp to CRP and H-NS
Publication
- 최근 5년간 대표 논문
- Duysak T, Afzal AR, Jung CH Determination of glutathione-binding to proteins by fluorescence spectroscopy.
- Biochem Biophys Res Commun. 2021 Jun;557:329-333.
- Duysak T, Nguyen LP, Jung CH Binding of Glutathione and ppGpp to Stringent Starvation Protein A (SspA) Bull. Kor. Chem. Soc. 2020 Sep;41(9):925-929
- Hoang HG, Tran TT, Jung CH The Activation of Glycerol Dehydrogenase from Escherichia coli by ppGpp. Bull. Kor. Chem. Soc. 2020 Feb;41(2):133-138.
- Nguyen TD, Jeong K, Ryu J, Jung CH A rapid screening of ligand binding by measuring intrinsic fluorescence changes of proteins. Bull. Kor. Chem. Soc. 2017 Sep;38(9):1028-1032