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| Department | Biomedical Sciences |
|---|---|
| Major Field of Research | Infectious Diseases, Biocalorimetry |
| strazvan@jnu.ac.kr | |
| Homepage |
Research interests
Stan Razvan’s lab is performing research into two avenues:
1. Molecular mechanisms underlying changes in monoclonal antibody binding affinity for viral and bacterial antigens during fever.
Fever is a regulated increase in core body temperature following infections, that aids in survival. While use of antipyretics may have severe adverse impact on patients suffering with sepsis, prolonged fever has nonetheless detrimental effects on human health.
We are investigating with biophysical, molecular dynamics and immunological techniques the mechanisms of thermal transfer at fever temperatures in immune complexes (collaborations with Prof. Choi Yoonjoo from Chonnam National University Medical School, South Korea, and with Prof. Maristela de Camargo from University of São Paulo, Brazil).
2. Mechanisms underlying transient molecular memory events following repeated stimulations.
- In vitro memory formation in protein complexes is essential for understanding cellular signaling and adaptation to environment, and is relevant for all aspects of immunology.
- We are investigating the effects of priming and conditioning on formation of protein complexes in vitro with two systems: immune complexes and enzymatic complexes. (collaboration with Dr. Aura Precupas from the Institute of Physical Chemistry, Romanian Academy of Science, Romania).
Publication
- 1. Bhat D, Stan R., Rundles C.R, Vogel C, de Camargo M.M. Chemical chaperones reverse early suppression of regulatory circuits during Unfolded Protein Response in B cells from CVID patients. Clinical and Experimental Immunology, 200, 1, 2020.
- 2. Stan, R.; Bhat, D.; de Camargo, M.M. Cellular adaptation relies on regulatory proteins having episodic memory. BioEssays. 42, 11, 2020.
- 3. Stan, R.; Soares, I.; Ferreira, L.C.S.; de Camargo, M.M. Febrile temperatures increase in vitro antibody affinity for malaria and dengue antigens. PLOS Neglected Tropical Diseases, 13, e0007239, 2019.
- 4. Stan, R.; de Camargo, M.M. Memory of Periodic Thermal Stimulation in an Immune Complex. ChemistrySelect, 4, 3325-3328, 2019.
- 5. Stan, R.; Bonin C.; Porto R.; Soriano F.; de Camargo, M.M. Increased grp78 transcription is correlated to reduced tlr4 transcription in patients surviving sepsis. Clinical and Experimental Immunology, 198, 273-280, 2019.
- 6. Bhat D., Ghiol DE., Costache A., Stan, R. Current experimental methods for analyzing protein-protein interactions, Archives of Microbiology and Immunology, 78, 1, 2019.
- 7. Stan, R.; Soriano F.; de Camargo, M.M. A mathematical model relates intracellular TLR4 oscillations to sepsis progression. BMC Reports, 11, 462, 2018.
- 8. Stan, R.; Appel, J.; Sanghamithra, N.J.M. Electrochemistry of Azurin H117G in Complex with Molecular Wires on Edge Plane Pyrolytic Graphite Electrodes. Electroanalysis, 30, 1091, 2018.
- 9. Stan, R.; Silva R.L.; de Camargo M.M.; Human GRP78 affinity towards its signaling partners Ire1α and PERK is differently modulated by an unfolded protein client. Biochemical and Biophysical Research Communications, 487, 375, 2017.
- 10. Stan, R.; Kros, A; Akkilic, N; Sanghamithra, N.J.M.; Appel, J. Direct wiring of the azurin redox center to gold electrodes investigated by protein film voltammetry. Journal of electroanalytical chemistry, 787, 14, 2017.